Journal: Antioxidants

Article Title: Therapeutic Potential of Cranberry Proanthocyanidins in Addressing the Pathophysiology of Metabolic Syndrome: A Scrutiny of Select Mechanisms of Action

doi: 10.3390/antiox14030268

Figure Lengend Snippet: Effect of PAC on insulin resistance, fasting lipid profile, lipoprotein composition, and inflammatory circulating biomarkers. At weeks 0, 6, and 10, ( A ) fasting glycemia and ( B ) fasting insulinemia was measured, enabling the calculation of the ( C ) Homeostatic model assessment of insulin resistance (HOMA-IR). At weeks 0, 6, 10, and 12, ( D ) fasting triglyceridemia and ( E ) fasting total cholesterolemia were measured. Results are presented as means ± SEM for n = 6–12 mice/group. At week 12, the lipid profile was further investigated to determine levels of ( F ) HDL-cholesterol and ( G ) non-HDL-cholesterol. Results are presented as means ± SEM for n = 4 pooled plasma/group. At week 12, plasma from n = 8 mice/group was collected and pooled for lipoprotein determination. Isolated fractions were first obtained via fast-protein liquid chromatography (FLPC) and then were analyzed to reveal content in ( H ) total cholesterol and ( I ) esterified cholesterol, as described in Materials and Methods. Circulating ( J ) adiponectin was measured at week 10. Circulating ( K ) interleukin-1-alpha (IL1A), ( L ) Monocyte Chemoattractant Protein-1 (MCP1), and ( M ) Tumor Necrosis Factor-alpha (TNFA) were obtained at week 12. Results are presented as means ± SEM for n = 6–8 mice/group. * p < 0.05, ** p < 0.01, *** p < 0.001 vs. Chow; # p < 0.05, ## p < 0.01, ### p < 0.001 vs. HFHF mice.

Article Snippet: Plasma samples ( n = 8 mice/group) obtained at week 10 were used to measure high molecular weight (HMW) adiponectin concentrations with the Mouse HMW and Total Adiponectin ELISA kit (ALPCO, Salem, NH, USA).

Techniques: Clinical Proteomics, Isolation, Fast Protein Liquid Chromatography